![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K4743030:Design
Designed by: Jen Hsien, Liu Group: iGEM23_PTSH-Taiwan (2023-09-16)
Bacillus subtilis Hydrogen sulfide based kill switch
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 464
Illegal AgeI site found at 1623 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 677
Design Notes
Sometimes it will be hard for companies to make up this sequence, because it has palindrome at the ROX sequence
Source
NO
References
1.Shimizu, T., Shen, J., Fang, M., Zhang, Y., Hori, K., Trinidad, J. C., Bauer, C. E., Giedroc, D. P., & Masuda, S. (2017). Sulfide-responsive transcriptional repressor SqrR functions as a master regulator of sulfide-dependent photosynthesis. Proceedings of the National Academy of Sciences of the United States of America, 114(9), 2355–2360. https://doi.org/10.1073/pnas.1614133114